ICAR Research Data Repository for Knowledge Management
Production of Polyclonal Antibody for Oxytetracycline and their use in Lateral Flow Assay.
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Production of Polyclonal Antibody for Oxytetracycline and their use in Lateral Flow Assay.
Not Available |
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| Creator |
Naik, L. Lata, K.; Sharma, R.; Mann, B. and Rajput, Y.S.
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| Subject |
Oxytetracycline, Milk, Antibiotic residue, Lateral flow assay.
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| Description |
Not Available
A new synthesis way of haptens for oxytetracycline (OTC) with Keyhole limpet hemocyanin (KLH) was demonstrated. This hapten conjugate (OTC-KLH) were used to successfully raise polyclonal antibodies against OTC. Utilizing this antibodies a rapid, user friendly and sensitive lateral flow assay (LFA) was designed to detect the OTC in milk system. From this assay results could be accomplished within 5 min. without the need of any equipment. The visual detection limit was 30 μg/kg. Proposed concept of OTC antibody production found to be a simple and reliable method for LFA. Thus, the developed assay could be used in the milk system to carry out onsite screening for OTC, from the beginning of the food chain to improve commercial trade and to uphold the consumer safety. Not Available |
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| Date |
2021-08-26T08:29:53Z
2021-08-26T08:29:53Z 2014-12-28 |
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| Type |
Research Paper
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60740 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Not Available
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