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An expedient ion chromatography based method for high‐throughput analysis of phytic acid in groundnut kernels

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Title An expedient ion chromatography based method for high‐throughput analysis of phytic acid in groundnut kernels
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Creator Aman Verma
Sushmita Singh
L.K. Thawait
Mahesh K. Mahatma
A.L. Singh
 
Subject Absorption
Phytic acid
 
Description Not Available
A study was made to expedite ion chromatography method using IonPac analytical column and self regenerating anion suppressor for phytic acid determination in groundnut seeds and compared with widely adopted spectrophotometric method based on enzymatic hydrolysis. The Ion Chromatography method equipped with AG11 guard and AS11 analytical columns in isocratic mode using 0.65 mM NaOH mobile phase at 1 mL min-1 flow rate showed a sharp peak for phytic acid with a retention time of 2.42±0.2 min. The peak area were plotted v/s concentration showed linearity with R2 value of 0.997, detection limit of 0.028 mg L-1 and recovery of 98% as against R2 value of 0.988 and detection limit of 0.065 mg L-1 in spectrophotometric method. The study clearly demonstrates that Ion Chromatography method was more accurate with better detection limit than spectrophotometry. Also, this method provides powerful handling with lesser reagent requirement due to combined eluent generation and self-regenerating suppression.
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Date 2022-11-07T11:02:48Z
2022-11-07T11:02:48Z
2022-11-01
 
Type Research Paper
 
Identifier Aman Verma, Sushmita Singh, Lokesh K. Thawait, Mahesh K. Mahatma and AL Singh (2022) An expedient ion chromatography based method for high‐throughput analysis of phytic acid in groundnut kernels. Journal of Food Science and Technology. 59: 4479-4486. https://doi.org/10.1007/s13197-022-05527-9
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http://krishi.icar.gov.in/jspui/handle/123456789/74872
 
Language English
 
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Publisher Not Available