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In silico assisted identification of peppery aroma compound ‘rotundone’ backbone genes from black pepper

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Title In silico assisted identification of peppery aroma compound ‘rotundone’ backbone genes from black pepper
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Creator Johnson George Kokkat, Sreekumar Shelvy, Abdulkabeer Muhammed Fayad, Ahammed Shabeer TP, Palaniyandi Umadevi, Rajaram Kale, Ulavappa Basavanneppa Angadi, Mir Asif Iquebal, Sarika Jaiswal, Anil Rai, Dinesh Kumar
 
Subject Piper nigrum
alpha quaeine synthase
alpha quaeine oxidase
berry transcriptome
metabolite profiling
 
Description Not Available
Rotundone, an oxygenated sesquiterpene compound, responsible for the peppery aroma. The importance of the rotundone in the flavor industry warrants search for the precursor genes in plants. We report in this study, the first on the identification of rotundone backbone genes viz., α-guaiene synthase & α-guaiene oxidase in black pepper. We identified the precursor genes of rotundone using berry transcriptome profiling. The metabolite profiling using head space mass spectrometry showed the presence of the direct precursor compounds for rotundone biosynthesis in black pepper berries. The identification of the genes & compounds of the guaiene skeleton is expected to help in bioprospecting of black pepper varieties & also in recombinant production of the aroma compound.
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Date 2021-07-23T05:52:58Z
2021-07-23T05:52:58Z
2021-02-10
 
Type Article
 
Identifier Kokkat, Johnson George, Sreekumar Shelvy, Abdulkabeer Muhammed Fayad, Ahammed Shabeer TP, Palaniyandi Umadevi, Rajaram Kale, Ulavappa Basavanneppa Angadi et al. "In silico assisted identification of peppery aroma compound ‘rotundone’backbone genes from black pepper." Journal of Biomolecular Structure and Dynamics (2021): 1-7.
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http://krishi.icar.gov.in/jspui/handle/123456789/49451
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis