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Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus

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Title Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus
Not Available
 
Creator A. I. Bhat
A. Siljo
M.V.Jiby
C.K.Thankamani
P.A.Mathew
 
Subject black pepper, indexing, PCR, Piper nigrum, Piper yellow mottle virus
 
Description Not Available
A polymerase chain reaction (PCR) based method was used for indexing 845 plants representing 14 popular varieties of black pepper (Piper nigrum) showing no visible external symptoms (apparently healthy) for Piper yellow mottle virus (PYMoV), among which 694 plants were positive (82%) for PYMoV. The percentage of infected plants ranged from 59% to 100% in different varieties; 100% of the tested plants of Panniyur-6 were infected with PYMoV and Sreekara had the least number of infected plants (59%). Some of the indexed PCR positive plants exhibited visible symptoms such as mild chlorosis, yellow specks and mottling in 1-3 months after testing. The study showed that indexing by PCR successfully detected PYMoV in infected black pepper plants showing no visible symptoms. The method can be used in certification programmes to identify PYMoV-free plants for further propagation
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Date 2021-07-29T05:32:32Z
2021-07-29T05:32:32Z
2009-06-01
 
Type Research Paper
 
Identifier Bhat, A.I., Siljo, A., Jiby, M.V., Thankamani, C.K. and Mathew. P.A. 2009. Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus. Journal of Spices and Aromatic Crops, 18: 28-32.
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http://krishi.icar.gov.in/jspui/handle/123456789/50957
 
Language English
 
Relation Not Available;
 
Publisher Indian Society for Spices