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Title	Micropropagation of Mangifera indica L. cv. Kurukkan through somatic embryogenesis Not Available

Creator	Mishra, M. Shree, Y., Pati, R., Seal,S., Shukla,N.,Kamle,M., Chandra, R. and Srivastava,A.

Subject	Abiotic stress, Kurakkan, Mangifera indica, Polyembryonic, Rootstock, Salt tolerant, Somatic embryogenesis

Description	Not Available Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52µM 2,4- D, 0.05% malt extract and 13.78µM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26µM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15 o C. Somatic embryos were matured on modified MS medium fortified with 0.38µM ABA, 0.57µM IAA and 30.30µM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68µM NAA, 11.60µM kinetin and 2736.9µM glutamine. Not Available

Date	2021-08-13T06:40:07Z 2021-08-13T06:40:07Z 2010-01-01

Type	Article

Identifier	Not Available Not Available http://krishi.icar.gov.in/jspui/handle/123456789/56245

Language	English

Relation	Not Available;

Publisher	Not Available

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