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Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton)

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Title Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton)
Not Available
 
Creator Chaturvedi Preeti
Dhanik Meenakshi
Pande Veena
Pande Amit
 
Subject Liver-expressed antimicrobial peptide (LEAP), Antimicrobial peptides (AMPs), Hepcidin, Innate immunity, Microbes
 
Description Not Available
Antimicrobial peptides extensively expressed in vertebrates, and invertebrates have assorted mechanisms of action and protect against pathogens. Liver-expressed antimicrobial peptide (LEAP) is a multifunctional cysteine-rich peptide that provides protection against infectious pathogens besides iron regulation. We have identified and cloned cDNA encoding LEAP from Tor putitora (TP-LEAP) as 440 bp fragment, with an open reading frame (ORF) of 279 nucleotides. The ORF encodes a pre-propeptide of 93 and a mature peptide of 25 amino acids. 3D model of the mature TP-LEAP, predominantly a β sheet, was generated and validated. Motif analysis and docking of TP-LEAP with iron transporter ferroportin revealed that the disulfide bonds are responsible for a stable structure besides interaction with ferroportin. Arg8 and Cys11, of mature TP-LEAP, formhydrogen bonds with Asn332 and Thr323 with bond lengths of 2.48Å and 2.55Å, respectively. TP-LEAP was phylogenetically closely related to Schizothorax richardsonii and Cyprinus carpio.
Not Available
 
Date 2020-09-26T07:29:36Z
2020-09-26T07:29:36Z
2016-01-01
 
Type Research Paper
 
Identifier Chaturvedi, P., Dhanik, M. and Pande, A. (2016). Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton). Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 37(2):67-76. DOI:10.5958/0974-0147.2016.00013.1
0970-9320
http://krishi.icar.gov.in/jspui/handle/123456789/41631
 
Language English
 
Relation Not Available;
 
Publisher Indian Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases