Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton)
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Title |
Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton)
Not Available |
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Creator |
Chaturvedi Preeti
Dhanik Meenakshi Pande Veena Pande Amit |
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Subject |
Liver-expressed antimicrobial peptide (LEAP), Antimicrobial peptides (AMPs), Hepcidin, Innate immunity, Microbes
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Description |
Not Available
Antimicrobial peptides extensively expressed in vertebrates, and invertebrates have assorted mechanisms of action and protect against pathogens. Liver-expressed antimicrobial peptide (LEAP) is a multifunctional cysteine-rich peptide that provides protection against infectious pathogens besides iron regulation. We have identified and cloned cDNA encoding LEAP from Tor putitora (TP-LEAP) as 440 bp fragment, with an open reading frame (ORF) of 279 nucleotides. The ORF encodes a pre-propeptide of 93 and a mature peptide of 25 amino acids. 3D model of the mature TP-LEAP, predominantly a β sheet, was generated and validated. Motif analysis and docking of TP-LEAP with iron transporter ferroportin revealed that the disulfide bonds are responsible for a stable structure besides interaction with ferroportin. Arg8 and Cys11, of mature TP-LEAP, formhydrogen bonds with Asn332 and Thr323 with bond lengths of 2.48Å and 2.55Å, respectively. TP-LEAP was phylogenetically closely related to Schizothorax richardsonii and Cyprinus carpio. Not Available |
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Date |
2020-09-26T07:29:36Z
2020-09-26T07:29:36Z 2016-01-01 |
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Type |
Research Paper
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Identifier |
Chaturvedi, P., Dhanik, M. and Pande, A. (2016). Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton). Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 37(2):67-76. DOI:10.5958/0974-0147.2016.00013.1
0970-9320 http://krishi.icar.gov.in/jspui/handle/123456789/41631 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases
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