Characterization and phylogenetic analysis of ectoine biosynthesis genes from Bacillus halodurans
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Title |
Characterization and phylogenetic analysis of ectoine biosynthesis genes from Bacillus halodurans
Not Available |
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Creator |
Anburajan, L.
Joseph, T.C. Nirmala Thampuran Roswin James Visvanathan, C. Bansal, K. C. |
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Subject |
B. halodurans
Compatible solutes Salt stress Ectoine genes Osmoregulation |
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Description |
Not Available
Ectoine, a cyclic tetrahydropyrimidine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was confirmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved. Not Available |
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Date |
2021-08-05T09:37:50Z
2021-08-05T09:37:50Z 2008-07-16 |
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Type |
Research Paper
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Identifier |
Anburajan, L., Joseph, T. C., Nirmala Thampuran, Roswin James, Visvanathan, C. and Bansal, K. C. (2008) Characterisation and phylogenetic analysis ecotine biosynthesis genes from Bacillus halodurans. Archives Microbiol. 190: 481-487.
0302-8933 http://krishi.icar.gov.in/jspui/handle/123456789/53288 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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