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Characterization and phylogenetic analysis of ectoine biosynthesis genes from Bacillus halodurans

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Title Characterization and phylogenetic analysis of ectoine biosynthesis genes from Bacillus halodurans
Not Available
 
Creator Anburajan, L.
Joseph, T.C.
Nirmala Thampuran
Roswin James
Visvanathan, C.
Bansal, K. C.
 
Subject B. halodurans
Compatible solutes
Salt stress
Ectoine genes
Osmoregulation
 
Description Not Available
Ectoine, a cyclic tetrahydropyrimidine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was confirmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved.
Not Available
 
Date 2021-08-05T09:37:50Z
2021-08-05T09:37:50Z
2008-07-16
 
Type Research Paper
 
Identifier Anburajan, L., Joseph, T. C., Nirmala Thampuran, Roswin James, Visvanathan, C. and Bansal, K. C. (2008) Characterisation and phylogenetic analysis ecotine biosynthesis genes from Bacillus halodurans. Archives Microbiol. 190: 481-487.
0302-8933
http://krishi.icar.gov.in/jspui/handle/123456789/53288
 
Language English
 
Relation Not Available;
 
Publisher Springer