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Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance

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Title Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance
Not Available
 
Creator Joseph, T.C.
Anbu Rajan, L.
Nirmala Thampuran
Roswin James
 
Subject E. coli
Compatible solutes
Trehalose
Salt stress
otsA
otsB
 
Description Not Available
Trehalose (1-a-D-glucopyranosyl-1-a-D-glucopyranoside), a non-reducing disaccharide is a major compatible solute, which maintains fluidity of membranes and
protects the biological structure of organisms under stress.
In this study, trehalose-6-phosphate synthase (otsA) and
trehalose-6-phosphate phosphatase (otsB) genes encoding
for trehalose biosynthesis from Escherichia coli was cloned
as an operon and expressed in E. coli M15(pREP4). The
recombinant E. coli strain showed a threefold increase in
the activity of otsBA pathway enzymes, compared to the
control strain. The transgenic E. coli accumulated up to
0.86 mg/l of trehalose. The sequence of otsA and otsB
genes reported in this study contains several base substitutions with that of reported sequences in GenBank,
resulting in the altered amino acid sequences of the translated proteins.
Not Available
 
Date 2021-08-04T10:40:29Z
2021-08-04T10:40:29Z
2010-09
 
Type Research Paper
 
Identifier Joseph, T. C., Anbu Rajan, L., Nirmala Thampuran and Roswin James (2010) Functional characterization of Trehalose biosynthesis genes from E. coli: An osmolyte involved in stress tolerance, Mol. Biotechnol., 46(1): 20–25.
1073-6085
http://krishi.icar.gov.in/jspui/handle/123456789/53088
 
Language English
 
Relation Not Available;
 
Publisher Springer