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Influence of pH on the solubility and conformational characteristics of muscle proteins from mullet (Mugil cephalus)

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Title Influence of pH on the solubility and conformational characteristics of muscle proteins from mullet (Mugil cephalus)
Not Available
 
Creator Mukund Mohan
Dhanya Ramachandran
Sankar, T.V.
Anandan, R.
 
Subject Fish muscle
pH
Solubility
Reactive sulphydryls
Surface hydrophobicity
SDS-PAGE
 
Description Not Available
Mullet (Mugil cephalus) muscle homogenates were adjusted to different pH ranging from 2 to 12 and the proteins extracted were evaluated for changes in solubility and conformational characteristics viz. surface hydrophobicity and reactive sulphydryl groups. Altering the pH of muscle homogenate to acidic or alkaline increased protein solubility. The hydrophobicity of the proteins increased on exposure to extreme pH indicating unfolding. The reactive sulphydryl groups decreased at acidic and alkaline pH with the lowest at pH 4. When the pH of the muscle homogenates was brought back to the original pH (6.3), the protein solubility was found to decrease. Reactive sulphydryl groups and ANS hydrophobicity of the proteins increased on readjusting the pH resulting in a molten-globule state. The electrophortogram of the samples corresponded well with the observations. Alterations in functional properties of these modified proteins are an area of interest for commercial application.
Not Available
 
Date 2021-08-16T03:48:48Z
2021-08-16T03:48:48Z
2007-07
 
Type Research Paper
 
Identifier Mukund Mohan, Dhanya Ramachandran, Sankar, T. V. and Anandan, R. (2007) Influence of pH on the solubility and conformational characteristics of muscle proteins from mullet (Mugil cephalus). Process Biochem. 42: 1056-1062.
1359-5113
http://krishi.icar.gov.in/jspui/handle/123456789/56338
 
Language English
 
Relation Not Available;
 
Publisher Elsevier