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Development and Characterization of Novel Polymorphic Microsatellite Loci in Freshwater Mussel Lamellidens marginalis (Lamarck, 1819) Using Next Generation Sequencing

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Title Development and Characterization of Novel Polymorphic Microsatellite Loci in Freshwater Mussel Lamellidens marginalis (Lamarck, 1819) Using Next Generation Sequencing
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Creator Varshney, S., Sagwekar, M.,Pavan-Kumar, A., Das, R., Gireesh-Babu, P., Chaudhari, A., Krishna, G.
 
Subject Simple sequence repeats; bivalves; pearl mussel; stock management
 
Description Not Available
The freshwater bivalve Lamellidens marginalis is native to Southeast Asia and one of the most widely used mussels for pearl production in the region. No studies have been carried out on stock characterisation of L. marginalis due to the lack of molecular markers. In the present study, the genome of L. marginalis was sequenced at low coverage using an Illumina pairedend approach. A total of 268,403 contigs with a size range of 258–13,308 bp was assembled from 2.5 million high quality reads. The contigs were mined and 26, 877 simple sequence repeats (SSRs) were identified using MIcroSAtellite identification tool software. Among SSRs, di-nucleotide repeats (15,633 total) were more frequent than the tri- (6306), tetra- (4768), penta- (146) or hexa-nucleotide (24) repeats. Eighteen loci representing di- (eight), tri- (seven) and tetra- (four) nucleotide repeats were characterised using 30 individuals of L. marginalis.
All loci showed moderate to high Polymorphic Information Content values. There was no
linkage disequilibria between any pair of loci. Fourteen microsatellites were successfully
amplified in the closely related species Lamellidens corrianus. These polymorphic
microsatellite markers will be useful for genetic selection programs, as well as for studying the population and conservation genetics of L. marginalis and L. corrianus.
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Date 2022-06-16T04:25:38Z
2022-06-16T04:25:38Z
2020-08-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/72587
 
Language English
 
Relation Not Available;
 
Publisher Not Available