Nitrification potential of Achromobacter xylosoxidans isolated from freshwater finfish farms of Kerala, India
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Title |
Nitrification potential of Achromobacter xylosoxidans isolated from freshwater finfish farms of Kerala, India
Not Available |
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Creator |
Basha KA*, Joseph T, Lalitha KV, Prasad KP, D. Vineetha, G. Rathore, Gayatri Tripati
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Subject |
Sediment, PCR, 16SrDNA, Achromobacter xylosoxidans, sodium pyruvate, Hetertrophic nitrification
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Description |
Not Available
A heterotrophic strain ACM-1, isolated from Pangasius spp fish farms showed an ability to convert ammonium to nitrate under aerobic conditions. Based on the molecular phylogenetic analysis 16S rRNA gene sequence ACM-1 matched similarity to Achromobacter xylosoxidans (100%). ACM-1 showed amplification for Betaproteobacterial 16S AOB gene (465 bp) confirming the characteristic feature of ammonia oxidation. Sodium pyruvate (1%) was confirmed as the probable carbon source for the luxurious growth of Achromobacter xylosoxidans among the six carbon sources tested. At 30oC, 5% preculture and 120 rpm in shaker, ACM-1 could able to remove 100% ammonium within 36h. The ammonium removal rate was reported to be 1.3 NH4 + -N mg L -1 h -1 at 30oC. The rate of ammonium removal at different temperature (20oC, 25oC, 37oC) ranged between 0.9 to1.33 NH4 + -N mg L-1 h -1 . The nitrite produced during this experiment remained at constant rate and nitrate concentration increased after the initial hours of experiment. Therefore, the results demonstrate that Achromobacter xylosoxidans ACM-1 can remove ammonium in batch cultures and could be applied in aquaculture farms as a biological nitrifier. Not Available |
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Date |
2022-06-18T05:50:56Z
2022-06-18T05:50:56Z 2018-07-11 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/72883 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Excellent Publisher
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