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High Frequency Induction of Multiple Shoots and Plant Regeneration from Cotyledonary Nodal Explant of Mung Bean [Vigna radiata (L) Wilczek]

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Title High Frequency Induction of Multiple Shoots and Plant Regeneration from Cotyledonary Nodal Explant of Mung Bean [Vigna radiata (L) Wilczek]
Not Available
 
Creator S. K. Yadav
Maheswari Mandapaka
Vanaja M
 
Subject BAP
half cotyledonary node with intact cotyledon
mung bean
regeneration
 
Description Not Available
An efficient protocol for shoot bud induction and proliferation employing half cotyledonary node with intact cotyledon
explants derived from two-day-old seedlings of mung bean pre-conditioned on 6- benzylaminopurine (BAP) has been
achieved. Explants were cultured for four weeks each on MS B5 + 12.5 μM BAP and MS B5 + 5 μM BAP +0.05 μM α-
naphthaleneacetic acid (NAA ), respectively, as shoot bud induction and shoot elongation and proliferation media, gave the
best regeneration response. The removal of the pre-existing buds from explants at 12 days in shoot bud induction medium
led to enhanced regeneration response. Light microscopic observations on 14-day-old explants confirmed direct
organogenesis route of regeneration. Elongated shoots (>2 cm) excised from the regenerating cultures were successfully
rooted on half MS B5 medium containing 2.46 μM indolebutyric acid (IBA). About 90% of the rooted plantlets, efficiently
hardened in pots having soil and farm yard manure, flowered and produced pods with viable seeds upon reaching maturity.
Not Available
 
Date 2021-08-17T09:51:06Z
2021-08-17T09:51:06Z
2010-07-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/57602
 
Language English
 
Relation Not Available;
 
Publisher Not Available