High Frequency Induction of Multiple Shoots and Plant Regeneration from Cotyledonary Nodal Explant of Mung Bean [Vigna radiata (L) Wilczek]
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Title |
High Frequency Induction of Multiple Shoots and Plant Regeneration from Cotyledonary Nodal Explant of Mung Bean [Vigna radiata (L) Wilczek]
Not Available |
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Creator |
S. K. Yadav
Maheswari Mandapaka Vanaja M |
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Subject |
BAP
half cotyledonary node with intact cotyledon mung bean regeneration |
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Description |
Not Available
An efficient protocol for shoot bud induction and proliferation employing half cotyledonary node with intact cotyledon explants derived from two-day-old seedlings of mung bean pre-conditioned on 6- benzylaminopurine (BAP) has been achieved. Explants were cultured for four weeks each on MS B5 + 12.5 μM BAP and MS B5 + 5 μM BAP +0.05 μM α- naphthaleneacetic acid (NAA ), respectively, as shoot bud induction and shoot elongation and proliferation media, gave the best regeneration response. The removal of the pre-existing buds from explants at 12 days in shoot bud induction medium led to enhanced regeneration response. Light microscopic observations on 14-day-old explants confirmed direct organogenesis route of regeneration. Elongated shoots (>2 cm) excised from the regenerating cultures were successfully rooted on half MS B5 medium containing 2.46 μM indolebutyric acid (IBA). About 90% of the rooted plantlets, efficiently hardened in pots having soil and farm yard manure, flowered and produced pods with viable seeds upon reaching maturity. Not Available |
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Date |
2021-08-17T09:51:06Z
2021-08-17T09:51:06Z 2010-07-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/57602 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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