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Production of doubled haploids from rice hybrid KRH-2 through anther culture and their evaluation for agro-morphological traits

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Title Production of doubled haploids from rice hybrid KRH-2 through anther culture and their evaluation for agro-morphological traits
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Creator Kulkarni SR, Ulaganathan K, Sundaram RM, HariPrasad AS, Abdul Fiyaz R and Balachandran SM
 
Subject Rice androgenesis
Callus induction
Green plant regeneration
KRH-2
morpho-agronomic evaluation
 
Description Not Available
The present study was aimed to optimize conditions of anther culture and production of doubled haploids of a popular rice hybrid, KRH-2. Immature panicles collected from field grown donor plants were pre-treated at 8°C for eight days and plated on four different basic media for callus induction with different hormonal concentrations and supplements. N6 media with maltose as the carbon source, supplemented with 2 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg L-1 of kinetin and 10 mg L-1 of silver nitrate (designated as N6-4 medium) was found to give the highest callus induction (22.4%). The compact calli, which were transferred to MS regeneration medium supplemented with 2.5 mg L-1 of benzyl amino purine (BAP), 0.5 mg L-1 of kinetin and 1.5 mg L-1 of naphthalene acetic acid (NAA) recorded a good regeneration (25.4%). A total of 125 stable, doubled haploid lines (DHLs) were produced through spontaneous doubling of KRH-2 microspores. The lines showed significant variability with respect to key agro-morphological traits
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Date 2021-08-04T05:56:57Z
2021-08-04T05:56:57Z
2020-05-30
 
Type Article
 
Identifier Kulkarni SR, Ulaganathan K, Sundaram RM, HariPrasad AS, Abdul Fiyaz R and Balachandran SM (2020). Production of doubled haploids from rice hybrid KRH-2 through anther culture and their evaluation for agro-morphological traits. Journal of Rice Research 13: 33-50
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http://krishi.icar.gov.in/jspui/handle/123456789/52823
 
Language English
 
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Publisher Not Available