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RAPD and ISSR markers for molecular characterization of Grewiaoptiva: an important fodder tree of north western Himalayas

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Title RAPD and ISSR markers for molecular characterization of Grewiaoptiva: an important fodder tree of north western Himalayas
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Creator Not Available
 
Subject Fodder tree, Genetic diversity,Grewia optiva, ISSR, RAPD
 
Description Not Available
RAPD (random amplified polymorphic DNA) and ISSR
(inter simple sequence repeats) markers were used to
study DNA polymorphism of thirty Grewia optivagenotypes
raised from seeds collected from various districts of
Himachal Pradesh (India) and selected on the basis of
morphological parameters. In general, the genotypes
exhibited a very high level of molecular diversity and DNA
polymorphism. Using 25 RAPD and 18 ISSR primers
molecular diversity based on Jaccard’s similarity
coefficients among 30 genotypes ranged from 0.17-0.83
in RAPD assay and 0.30-0.84 in ISSR assay. RAPD
primers amplified more loci than ISSR primers. RAPD
and ISSR primers amplified 96.31 and 91.72%
polymorphic loci, respectively. The dendrogram derived
using the unweighted pair-group method with arithmetic
averaging (UPGMA) clustered the genotypes into different
groups reflecting geographical sub-structuring of the
genetic diversity. Genotype SO-12 (Kasauli) was found to
be the most divergent genotype which could be used for
number of combinations to be established in the seed
orchards and as a parent in hybridization programme
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Date 2018-11-13T09:33:43Z
2018-11-13T09:33:43Z
2015-08-01
 
Type Research Paper
 
Identifier Not Available
0971-2070
http://krishi.icar.gov.in/jspui/handle/123456789/10897
 
Language English
 
Relation Not Available;
 
Publisher Not Available