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Addition of Cholesterol-Loaded Cylodextrin Improves Cryosurvival of Buffalo Spermatozoa

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Title Addition of Cholesterol-Loaded Cylodextrin Improves Cryosurvival of Buffalo Spermatozoa
Not Available
 
Creator Kumar A.
Prasad J. K.
MAHAK SINGH
Rajoriya J.S
 
Subject Buffalo bull sperm
Cholesterol
Cyclodexterin
Cryopreservation
Post-thaw
 
Description Not Available
Cryopreservation helps in prolonging the storage of semen but
freezing and thawing damage the sperm because of alteration in the
structure and physiology of the sperm cell. Buffalo bull sperm were treated
with different levels of cholesterol loaded cyclodextrin (CLC) and frozen in
tris egg yolk glycerol diluents to determine the best suitable concentrations
of CLC that benefits Buffalo sperm cryosurvival. Semen collected from
Buffalo bull was diluted to 120×106 spermatozoa/ml by tris diluents and
divided into four groups; control (without CLC), 0.5, 1.0 and 2.0 mg
CLC/ml of diluted semen as Group I, II, III and IV respectively. At prefreeze
stage, the sperm treated with 2.0 mg CLC showed significantly
(P
Not Available
 
Date 2021-09-29T06:21:54Z
2021-09-29T06:21:54Z
2019-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/64795
 
Language English
 
Relation Not Available;
 
Publisher Not Available