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CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome

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Title CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome
Not Available
 
Creator Kutubuddin A. MollaYinong Yang
 
Subject CRISPR-Cas, Base Editing, Locus in Rice
 
Description Not Available
Base editing is a recently developed technology that enables us to install point mutation in the genome precisely. The fusion of nucleotide deaminase with Cas9 nickase gives rise to base editors. Like the conventional CRISPR-Cas9 technology, a single-guide RNA can direct a base editor to a particular locus to change one nucleotide to another. In this chapter, we describe a stepwise protocol to perform targeted base editing experiments in rice plants.
Not Available
 
Date 2021-01-01T12:12:42Z
2021-01-01T12:12:42Z
2020-07-01
 
Type Book
 
Identifier https://doi.org/10.1007/978-1-0716-0616-2_4
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/44431
 
Language English
 
Relation Not Available;
 
Publisher Springer