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Isolation and partial characterization of antibacterial lipopeptide produced by Paenibacillus polymyxa HKA-15 against phytopathogen Xanthomonas campestris pv. phaseoli M-5

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Title Isolation and partial characterization of antibacterial lipopeptide produced by Paenibacillus polymyxa HKA-15 against phytopathogen Xanthomonas campestris pv. phaseoli M-5
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Creator V Mageshwaran
S Walia
K Annapurna
 
Subject Antibacterial activity Characterization Lipopeptide Paenibacillus polymyxa Purification Xanthomonas campestris pv. phaseoli
 
Description Not Available
An antibacterial metabolite was isolated from
Paenibacillus polymyxa HKA-15, a soybean bacterial
endophyte. The purification of the crude metabolite from
Paenibacillus polymyxa HKA-15 was done by column
chromatography. In TLC, a spot with an Rf value of 0.86
(±0.02) from the purified fraction showed bioactivity
against Xanthomonas campestris pv. phaseoli M-5. In
SDS-PAGE, the purified antibiotic was separated in the
molecular weight range of 3.5 kDa. The exact molecular
weight of the active compound was identified as
1,347.7 Da using MS-MS analysis. Infra red spectrum and
1
H NMR analysis showed the presence of amino acids and
fatty acids in the active compound. The characterization of
the antibacterial compound revealed its lipopeptide nature.
In an agar diffusion assay, the crude metabolite showed a
broad spectrum of activity, being able to inhibit the growth
of the fungal pathogen, Rhizoctonia bataticola, Macrophomina phaseolina and Fusarium udum. A stronger
inhibition was observed against bacterial pathogens viz.,
X. campestris pv.phaseoli M-5, X. campestris pv. phaseoli
CP-1-1, Xanthomonas oryzae, Ralstonia solanacearum and
Micrococcus luteus.
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Date 2021-07-28T10:56:31Z
2021-07-28T10:56:31Z
2012-01-01
 
Type Article
 
Identifier Mageshwaran V, Suresh Walia and K Annapurna. 2012. Isolation and partial characterization of antibacterial lipopeptide produced by Paenibacillus polymyxa HKA-15 against phytopathogen Xanthomonas campestris pv. phaseoli M-5. World Journal of Microbiology and Biotechnology.28: 909-917
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http://krishi.icar.gov.in/jspui/handle/123456789/50814
 
Language English
 
Relation Not Available;
 
Publisher Springer