ICAR Research Data Repository for Knowledge Management
Real time PCR assay based on Topoisomerase II gene for detection of Fusarium udum.
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Real time PCR assay based on Topoisomerase II gene for detection of Fusarium udum.
Not Available |
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| Creator |
Yadav MK, Babu BK, Saxena AK, Singh BP, Singh K and Arora D.K. 2010
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| Subject |
Housekeeping genes SYBR green Melting curve Standard curve
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| Description |
Not Available
Fusarium wilt is an important soilborne disease of pigeonpea, caused by Fusarium udum. In this study, we have designed a real-time PCR assay for the detection of Fusarium udum from infected pigeonpea plants. Based on Topoisomerase-II gene sequence data from Fusarium udum and other related Fusarium species, a pair of primer was designed. The species-specific primers were tested in real-time PCR SYBR green assay. No increasing fluorescence signals exceeding the baseline threshold was observed with tested microbes, except Fusarium udum DNA. A single dissociation peak of increased fluorescence was obtained for the specific primers at melting temperature of 81.25°C. The real-time PCR showed a lowest detection of 0.1 pg genomic DNA. The assay was more sensitive, accurate and less time consuming for detection of Fusarium udum in infected plants root. Not Available |
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| Date |
2021-11-01T10:21:57Z
2021-11-01T10:21:57Z 2010-01-01 |
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| Type |
Research Paper
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/66733 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Springer
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