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LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane.

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Title LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane.
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Creator Shingote PR, Amitha Mithra SV, Sharma P, Devanna NB, Arora K, Holkar SK, Khan S, Singh J, Kumar S, Sharma TR, Solanke AU
 
Subject Genetic fidelity testing, IRAP, ISSR, Phylogeny, Somaclonal variation, Sugarcane
 
Description Not Available
Testing the genetic fidelity of micropropagated clones with DNA markers could curtail the losses caused by somaclonal variation in sugarcane tissue culture industry. Evaluation of 32 inter-retrotransposon amplified polymorphism (IRAP) markers and 100 inter-simple sequence repeats (ISSRs) in 47 sugarcane accessions identified 20 (8 IRAPs and 12 ISSRs) polymorphic markers representing 98 loci. IRAP system was superior to ISSR in terms of marker index (2.68 as against 1.76), resolving power (2.85 as against 1.98), and polymorphic loci per assay (6 compared to 4.1), except mean polymorphic information content (0.31 and 0.34). Further evaluation of the 20 polymorphic markers in testing the genetic fidelity of micropropagated sugarcane clones identified a variant clone by three primers of ISSR and one IRAP marker (UBC810, UBC813, UBC840, and LTR6149 + 3’LTR). The unique amplicons from the somaclonal variant were validated by sequencing. Based on the number of bands amplified and proportion of polymorphic loci, a set of six ISSR and four IRAP markers has been recommended to test clonal fidelity for sugarcane tissue culture industry.
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Date 2021-07-29T04:14:38Z
2021-07-29T04:14:38Z
2019-01-21
 
Type Research Paper
 
Identifier Shingote PR, Amitha Mithra SV, Sharma P, Devanna NB, Arora K, Holkar SK, Khan S, Singh J, Kumar S, Sharma TR, Solanke AU (2019) LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane. Molecular Breeding 39 (2), 25 (DOI: 10.1007/s11032-019-0931-5)
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http://krishi.icar.gov.in/jspui/handle/123456789/50892
 
Language English
 
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