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Isolation and expression analysis of EcbZIP17 from different finger millet genotypes shows conserved nature of the gene.

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Title Isolation and expression analysis of EcbZIP17 from different finger millet genotypes shows conserved nature of the gene.
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Creator Ramakrishna Ch, Sonam, Mohanty S, Reddy N, Padaria JC and Solanke AU
 
Subject Finger millet, bZIP transcription factor, ER stress, Heat stress, Conserved gene
 
Description Not Available
Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic stress tolerance. In this study, we report isolation and characterization of EcbZIP17, a group B bZIP transcription factor from a climate smart cereal, finger millet (Eleusine coracana L.). The genomic sequence of EcbZIP17 is 2662 bp long encompassing two exons and one intron with ORF of 1722 bp and peptide length of 573 aa. This gene is homologous to AtbZIP17 (Arabidopsis), ZmbZIP17 (maize) and OsbZIP60 (rice) which play a key role in endoplasmic reticulum (ER) stress pathway. In silico analysis confirmed the presence of basic leucine zipper (bZIP) and transmembrane (TM) domains in the EcbZIP17 protein. Allele mining of this gene in 16 different genotypes by Sanger sequencing revealed no variation in nucleotide sequence, including the 618 bp long intron. Expression analysis of EcbZIP17 under heat stress exhibited similar pattern of expression in all the genotypes across time intervals with highest upregulation after 4 h.
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Date 2021-07-29T04:24:43Z
2021-07-29T04:24:43Z
2017-09-21
 
Type Research Paper
 
Identifier Ramakrishna Ch, Sonam, Mohanty S, Reddy N, Padaria JC and Solanke AU (2017). Isolation and expression analysis of EcbZIP17 from different finger millet genotypes shows conserved nature of the gene. 3 Biotech. (doi:10.1007/s13205-017-0984-2)
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http://krishi.icar.gov.in/jspui/handle/123456789/50899
 
Language English
 
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Publisher Not Available