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Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH

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Title Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH
Not Available
 
Creator Ravindra Kumar
Vishwamitra Singh Baisvar
Basdeo Kushwaha
Gusheinzed Waikhom
Labee Thoidingjam
S Sanjabihari Singh
 
Subject Glossogobius giuris
Giemsa
AgNOR
CMA3
FISH
18S rDNA
 
Description Not Available
The Glossogobius giuris (Gobiidae), tank goby a species of goby fish, is native mainly to freshwater and
estuaries and has importance in the aquarium trade. It is classified as least concern under IUCN Red List. Live individuals of this species were collected from Loktak Lake in Manipur for cytogenetic investigation to reveal the nucleolar organizer region (NOR) through silver nitrate (AgNO3) and chromomycin A3 (CMA3) staining as well as DAPI staining and single-color fluorescence in situ hybridization (FISH) using 18S rDNA sequence as probe. Analysis of more than 50 metaphase chromosome complement (obtained from
colchicine–potassium chloride–Carnoy’s fixation–Giemsa staining procedures) showed the presence of 46 diploid chromosome numbers with all telocentric having fundamental arm number as 46 without any heteromorphic pair. One pairs of silver-stained NORs, situated terminally on the telocentric chromosome, were observed. Similarly, one pair of CMA3-positive sites were observed on the chromosome
that suggested abundance of GC-rich repetitive DNAs in this region. One pair of 18S rDNA positive sites was observed on the telocentric chromosomes using FISH. These karyological features can be useful markers in cytotaxonomy and conservation of this species.
Department of Biotechnology, Ministry of Science and Technology, Government of India, for financial support through Project Sanction No. BT/13/NE/TBP/2010
 
Date 2021-07-28T10:27:53Z
2021-07-28T10:27:53Z
2020-03
 
Type Research Paper
 
Identifier Kumar, R., Baisvar, V.S., Kushwaha, B., Waikhom, G., Thoidingjam, L. and Singh, S.S., 2020. Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 90(1), pp.221-226.
2250-1746 (web)
0369-8211 (print)
http://krishi.icar.gov.in/jspui/handle/123456789/50784
 
Language English
 
Relation Not Available;
 
Publisher Springer India