PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates
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Title |
PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates
Not Available |
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Creator |
Mishra, S. S.
Brahmane, Manoj Mishra, S.K.. |
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Subject |
PCR
DNA |
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Description |
Not Available
V. parahaemolyticus isolates from fish and shrimp P. monodon were screened using PCR and 623 bp product could be visualized in positive cases. The selected isolates were further characterized using protein profile analysis on 11% SDS-PAGE and DNA restriction profile analysis. Variable protein bands on SDS-PAGE indicated antigenic variation among V. parahaemolyticus isolates. EcoRI, BamHI and Haelll were found unsuitable for DNA-RE analysis but Hind III digestion revealed numerous DNA fragments in the range of 15.0 kb to 0.5 kb with variation among isolates. Not Available |
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Date |
2021-07-29T04:41:14Z
2021-07-29T04:41:14Z 2009-01-01 |
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Type |
Research Paper
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Identifier |
Mishra, Sudhansu S. & Brahmane, Manoj & Mishra, S.K.. (2009). PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates. Indian Veterinary Journal. 86. 229-233.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/50931 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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