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PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates

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Title PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates
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Creator Mishra, S. S.
Brahmane, Manoj
Mishra, S.K..
 
Subject PCR
DNA
 
Description Not Available
V. parahaemolyticus isolates from fish and shrimp P. monodon were screened using PCR and 623 bp product could be visualized in positive cases. The selected isolates were further characterized using protein profile analysis on 11% SDS-PAGE and DNA restriction profile analysis. Variable protein bands on SDS-PAGE indicated antigenic variation among V. parahaemolyticus isolates. EcoRI, BamHI and Haelll were found unsuitable for DNA-RE analysis but Hind III digestion revealed numerous DNA fragments in the range of 15.0 kb to 0.5 kb with variation among isolates.
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Date 2021-07-29T04:41:14Z
2021-07-29T04:41:14Z
2009-01-01
 
Type Research Paper
 
Identifier Mishra, Sudhansu S. & Brahmane, Manoj & Mishra, S.K.. (2009). PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates. Indian Veterinary Journal. 86. 229-233.
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http://krishi.icar.gov.in/jspui/handle/123456789/50931
 
Language English
 
Relation Not Available;
 
Publisher Not Available