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Calcium phosphate nanoparticle prepared with foot and mouth disease virus P1-3CD gene construct protects mice and guinea pigs against the challenge virus

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Title Calcium phosphate nanoparticle prepared with foot and mouth disease virus P1-3CD gene construct protects mice and guinea pigs against the challenge virus
Not Available
 
Creator Dechamma Hosur Joyappa
C. Ashok Kumar
N. Banumathi
Golla Rama Reddy
Veluvarthy V.S. Suryanarayana
 
Subject Foot and mouth disease
Calcium phosphate nanoparticle
DNA vaccine
Immunity
 
Description Not Available
Calcium phosphate nanoparticles provide safe and easily manufactured vaccine adjuvant and delivery system for DNA vaccines. In the present study FMDV “O” P1-3CD DNA vaccine was encapsulated in calcium phosphate nanoparticles of size 50–100 nm diameters. The maximum loading and entrapment efficiency of nanoparticles were studied by spectrophotometer, as well as agarose gel electrophoresis. In vitro transfection efficiency of these calcium phosphate nanoparticles was found to be as good as commercial transfecting reagent lipofectamine. In vivo analysis of the calcium phosphate nanoparticle P1-3CD (CaPNP1-3CD) FMDV “O” vaccine in mice and guinea pigs could induce significant cell mediated and humoral immune response. Also, immunized mice and guinea pigs were protected against the challenge virus.
Not Available
 
Date 2023-05-12T05:05:23Z
2023-05-12T05:05:23Z
2009-10-20
 
Type Research Paper
 
Identifier Joyappa DH, Kumar CA, Banumathi N, Reddy GR, Suryanarayana VV. Calcium phosphate nanoparticle prepared with foot and mouth disease virus P1-3CD gene construct protects mice and guinea pigs against the challenge virus. Vet Microbiol. 2009 Oct 20;139(1-2):58-66. doi: 10.1016/j.vetmic.2009.05.004. Epub 2009 May 19. PMID: 19505774.
0378-1135
http://krishi.icar.gov.in/jspui/handle/123456789/77187
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER