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Immunological evaluation of mannosylated chitosan nanoparticles based foot and mouth disease virus DNA vaccine, pVAC FMDV VP1-OmpA in guinea pigs.

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Title Immunological evaluation of mannosylated chitosan nanoparticles based foot and mouth disease virus DNA vaccine, pVAC FMDV VP1-OmpA in guinea pigs.
 
Creator Nanda RK, Hajam IA, Edao BM, Ramya K, Rajangam M, Chandra Sekar S, Ganesh K, Bhanuprakash V, Kishore S.
 
Subject ELISA; FMD DNA vaccine; Mannosylated chitosan nanoparticles delivery; OmpA; Protection; SNT; Salmonella typhimurium.
 
Description A DNA vaccine for foot and mouth disease (FMD) based on mannosylated chitosan nanoparticles was evaluated in guinea pigs. The DNA construct was comprised of FMD virus full length-VP1 gene and outer membrane protein A (Omp A) gene of Salmonella typhimurium as a Toll-like receptor (TLR)-ligand in pVAC vector. Groups of guinea pigs immunized either intramuscularly or intra-nasally were evaluated for induction of virus neutralizing antibodies, Th1(IgG2) and Th2 (IgG1) responses, lymphocyte proliferation, reactive nitrogen intermediate production, secretory IgA for naso-mucosal immune response and protection upon homotypic type O virulent FMD virus challenge. The results indicate the synergistic effect of OmpA on the immunogenic potential of FMD DNA vaccine construct delivered using mannosylated chitosan nano-particles by different routes of administration. These observations suggest the substantial improvement in all the immunological parameters with enhanced protection in guinea pigs.
 
Date 2023-05-12T09:38:46Z
2023-05-12T09:38:46Z
2014-05-01
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77216
 
Language English
 
Publisher Elsevier