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Dietary arginine attenuates hypoxia-induced HIF expression, metabolic responses and oxidative stress in Indian Major Carp, Cirrhinus mrigala.

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Title Dietary arginine attenuates hypoxia-induced HIF expression, metabolic responses and oxidative stress in Indian Major Carp, Cirrhinus mrigala.
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Creator Not Available
 
Subject Mrigal HIF 1α Lipid peroxidation Antioxidant enzymes Erythrocyte fragility RBC count
 
Description Not Available
Hypoxia is a common stressor in aquaculture systems, which causes severe physiological disturbances, ultimately
leading to mortality or reduced productivity. Arginine, as a precursor of NO, has a role in enhancing oxygen
delivery. Thus, an experiment was conducted to evaluate the effect of dietary arginine (Arg) in Cirrhinus mrigala
exposed to hypoxia. The fish were fed with different levels of arginine for 60 days and exposed for 72 h to a
sublethal level of hypoxia (0.50 ± 0.16 mg/L dissolved oxygen [DO]). The six treatment groups with three
replicates were N0 (0% Arg + Normoxia), H0 (0% Arg + Hypoxia), N0.7 (0.70% Arg + Normoxia), H0.7 (0.70%
Arg + Hypoxia), N1.4 (1.40% Arg + Normoxia), H1.4 (1.40% Arg + Hypoxia). Eighteen experimental units with
twelve animals (5.8 ± 0.18 g) each were used for the trial.The results indicated that supplementation of arginine
at 0.7 and 1.4% enhanced the hypoxia tolerance time, although the high dose (1.4%) did not yield any further
increments. The exposure to hypoxia up-regulated Hypoxia Inducible Factor (HIF)-1α mRNA expression and
supplementation of arginine significantly decreased hypoxia induced up-regulation of HIF at 1.4%. Arginine
supplementation partially or completely normalised the hypoxia induced changes in the metabolic enzymes of
C. mrigala. The fish exposed to hypoxic conditions exhibited significantly higher (P < 0.05) lipid peroxidation
levels than those maintained under normoxic conditions, while arginine feeding significant in reducing lipid
peroxidation. Antioxidant enzyme activities were significantly higher (P < 0.05) in hypoxia-exposed carp,
indicating increased oxidative stress during the hypoxic exposure, that was improved in Arg-supplemented
groups. However, arginine did not modulate erythrocyte countsalthough itreduced the erythrocyte fragility.
We conclude arginine supplementation is effective in ameliorating hypoxia induced metabolic alterations and
improving antioxidant defences in fish.
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Date 2023-05-16T04:09:00Z
2023-05-16T04:09:00Z
2022-01-10
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77416
 
Language English
 
Relation Not Available;
 
Publisher Elsevier