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Development of a monoclonal antibody based Competitive ELISA for the detection of Rinderpest antibodies

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Title Development of a monoclonal antibody based Competitive ELISA for the detection of Rinderpest antibodies
 
Creator R P Singh
B P Sreenivasa
P Dhar
R N Roy
S K Bandyopadhyay
 
Subject competitive enzyme-linked immunosorbent assay
monoclonal antibody
rinderpest
 
Description Not Available
A competitive enzyme-linked immunosorbent assay has been standardised for the detection of antibodies to rinderpest virus in sera from cattle, sheep and goats. The test uses a neutralising monoclonal antibody (MAb) directed against the haemagglutinin protein of rinderpest virus. The test is specific for rinderpest, as it failed to detect antibodies to peste des petits ruminants virus in convalescent goat sera. A 45% inhibition of the binding of the MAb to the antigen was used as the cut-off point for deciding the rinderpest status of the test samples. The specificity and sensitivity of the test and the stability of the test reagents were determined and compared to the results obtained using a commercial kit with approximately 1,200 serum samples from cattle, sheep and goats in India. The current test compared very well with the commercial kit. The test is expected to be extremely useful for sero-monitoring and sero-surveillance of rinderpest in countries which are actively pursuing a rinderpest eradication programme.
Not Available
 
Date 2023-05-22T06:02:31Z
2023-05-22T06:02:31Z
2000-07-10
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77701
 
Language English
 
Relation Not Available;
 
Publisher Revue scientifique et technique (International Office of Epizootics)