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Genetic identity between Bhadawari and Murrah breeds of Indian Buffaloes (Bubalus bubalis) using RAPD-PCR

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Title Genetic identity between Bhadawari and Murrah breeds of Indian Buffaloes (Bubalus bubalis) using RAPD-PCR
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Creator Saifi, H. W.
Bhushan, B.
Kumar S
Kumar P
Patra B N
Sharma A
 
Subject Bhadawari and Murrah Buffaloes, Band Frequency, Genetic Identity Index, Mean Average Percentage Difference, RAPDPCR
 
Description Not Available
Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out with a
battery of 11 random decamer primers to study band frequency (BF), genetic identity index (I) and mean average percentage difference
(MAPD) between Bhadawari and Murrah breeds of buffalo. The primers OPA04 and BG15 resolved a band of 460 bp, which was
present only in animals of Bhadawari breed. Whereas, the primers OPA14, BG27 and BG28 produced Murrah specific fragments of
sizes 730 bp and 1,230 bp, respectively. The estimate of genetic identity index was highest (0.845) with the primer OPA01 and the
lowest (0.479) with the primer BG27. The genetic identity index pooled over the primers was 0.596±0.037 between these two breeds.
The highest MAPD estimate (53.9) between the two breeds was obtained with the primer BG27 and the lowest (14.3) with the primer
OPA01. It might be concluded that the genetic identity index between these two breeds calculated on the basis of BF showed moderate
level of genetic identity with the primers employed. MAPD calculated on the basis of uncommon bands also demonstrated lower to
medium level of genetic difference between Bhadawari and Murrah breeds of buffalo.
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Date 2023-05-22T06:01:46Z
2023-05-22T06:01:46Z
2004-01-01
 
Type Article
 
Identifier Saifi, H. W., Bhushan, B., Kumar, S., Kumar, P., Patra, B. N., & Sharma, A. (2004). Genetic identity between Bhadawari and Murrah breeds of Indian buffaloes (Bubalus bubalis) using RAPD-PCR. Asian-Australasian Journal of Animal Sciences, 17(5), 603-607.
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http://krishi.icar.gov.in/jspui/handle/123456789/77700
 
Language English
 
Relation Not Available;
 
Publisher Animal Bioscience